Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ (Jul 2011)
Probiotic Potential of Lactic Acid Bacteria Isolated from Breast Milk
Abstract
Background: Probiotic is viable microbe agent that beneficially affects the host possibly by improving the balance of the indigenous microflora. Many probiotic bacteria have been used as alternative treatment of some intestinal diseases such as diarrhoea, irritable bowel syndrome. Although probiotic strains can be isolated from many sources; for human applications the main criteria is being human origin.Breast milk is an important nutrient source for neonates. Lots of studies showed that this fluid has beneficial effects on the health of neonates. One reason of being beneficial is explaining by the microflora of human breast milk including beneficial lactic acid bacteria.Objectives: This work aimed to isolating and screening of potential probiotic properties of lactic acid bacteria strains from breast milk (milk of healthy mothers).Methods: Lactobacillus was isolated by MRS medium supplemented with CaCO3 to detect lactic acid. Probiotic characteristics were tested according to the guidelines of WHO/ FAO, which include: producing extracellular enzymes, resistance to low pH and bile salts, and safety aspects. Finally, these strains were identified according to Prokaryote – A Handbook on the Biology of Bacteria.Results: From 57 isolated strains from breast milk we selected 5 strains that showed antimicrobial activity against food poisoning bacteria, ability of producing extracellular enzymes and high ratio of L-lactic/D-lactic acid, resistance to low pH and bile salts, nonhemolytic and sensitive to tested antibiotics. The selected strains were identifed as species belong to Lactobacillus genus.Conclusion: In this work, 5 Lactobacillus strains have been selected and identified that meet the requirements for in vitro probiotic characteristic and safety tests according to the guidelines of WHO/ FAO. The results also showed that, breast milk is a source of potential probiotic strains.
