Nature Communications (Jan 2022)
53BP1 regulates heterochromatin through liquid phase separation
- Lei Zhang,
- Xinran Geng,
- Fangfang Wang,
- Jinshan Tang,
- Yu Ichida,
- Arishya Sharma,
- Sora Jin,
- Mingyue Chen,
- Mingliang Tang,
- Franklin Mayca Pozo,
- Wenxiu Wang,
- Janet Wang,
- Michal Wozniak,
- Xiaoxia Guo,
- Masaru Miyagi,
- Fulai Jin,
- Yongjie Xu,
- Xinsheng Yao,
- Youwei Zhang
Affiliations
- Lei Zhang
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Xinran Geng
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Fangfang Wang
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
- Jinshan Tang
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
- Yu Ichida
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Arishya Sharma
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Sora Jin
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Mingyue Chen
- National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering, Hubei University of Technology
- Mingliang Tang
- College of Life Sciences, Wuhan University
- Franklin Mayca Pozo
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Wenxiu Wang
- National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering, Hubei University of Technology
- Janet Wang
- Department of Genetics and Genome Sciences, Case Western Reserve University, School of Medicine
- Michal Wozniak
- Department of Pharmacology and Toxicology, Wright State University
- Xiaoxia Guo
- National 111 Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering, Hubei University of Technology
- Masaru Miyagi
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- Fulai Jin
- Department of Genetics and Genome Sciences, Case Western Reserve University, School of Medicine
- Yongjie Xu
- Department of Pharmacology and Toxicology, Wright State University
- Xinsheng Yao
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University
- Youwei Zhang
- Department of Pharmacology, Case Comprehensive Cancer Center, Case Western Reserve University, School of Medicine
- DOI
- https://doi.org/10.1038/s41467-022-28019-y
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
The 53BP1 protein is well-known for its involvement in double-strand break (DSB) repair. Here the authors show 53BP1 undergoes significant liquid-liquid phase separation (LLPS) to play a role in heterochromatin maintenance and identify distinct domains/residues that function in DSB repair versus LLPS.
