Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro

Linjing Xia; Xiaofei Liu; Huiyuan Guo; Hao Zhang; Jun Zhu; Fazheng Ren

Journal of Functional Foods (Jan 2012)

Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro

Linjing Xia,
Xiaofei Liu,
Huiyuan Guo,
Hao Zhang,
Jun Zhu,
Fazheng Ren

Affiliations

Linjing Xia: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, PR China
Xiaofei Liu: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, Beijing 100083, PR China
Huiyuan Guo: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, PR China
Hao Zhang: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, PR China
Jun Zhu: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, PR China
Fazheng Ren: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, PR China; Corresponding author at: College of Food Science & Nutritional Engineering, China Agricultural University, P.O. Box 287, No. 17 Qinghua East Road, Haidian, Beijing 100083, PR China. Tel./fax: +86 10 62736344.

Journal volume & issue: Vol. 4, no. 1
pp. 294 – 301

Abstract

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The crude polysaccharide from the stem of Dendrobium officinale (DOP) was obtained by water extraction and ethanol precipitation. Two fractions of DOP, DOP-1 and DOP-2, were fractioned by DEAE cellulose and Sephacryl S-400 gel filtration column chromatography. Their molecular weights and monosaccharide compositions were studied. The immunomodulatory activity of DOP, DOP-1 and DOP-2 was evaluated with in vitro cell models. The results showed that the 533.7 kDa DOP-1 and 159.5 kDa DOP-2 were mainly composed of mannose and glucose. Both DOP and its purified fractions significantly (P < 0.05) promoted splenocyte proliferation, enhanced natural killer cell-mediated cytotoxicity, and increased the phagocytosis and nitric oxide production of macrophages. Moreover, these two fractions caused a significant (P < 0.05) stimulation of cytokine secretion of both splenocytes and macrophages. These findings suggest that Dendrobium officinale polysaccharides should be explored as novel potential immunomodulators.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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