Human papilomavirus genotype distribution in women from Montenegro

Abstract

Introduction. The distribution of HPV genotypes in women can aid in the selection of an appropriate screening test for detection of HPV infection. For example, identification of particular HPV genotypes strains may reveal patients at high-risk. The purpose of this study was to determine the range and frequency of HPV genotypes in women in Montenegro. Methods. Cervical smears were taken at the Gynecological Department of the Clinical Center in Podgorica from women with clinical signs of HPV infection. HPV genotypes were determined using PCR amplification and enzyme restriction. PCR products were amplified with group-specific primers MY09/MY11 and restricted with seven different endonucleases. Patterns of restriction fragment length polymorphism (RFLP) were compared to predefined standard patterns. Results. We tested a total of 189 women and found HPV infection in 1/5 of them (20%). Among those infected (38 HPV DNA positive women), there were 16 different HPV genotypes. Genotyping of this group showed that a third of them had HPV genotype 16. The second most frequent HPV infection was with HPV genotype 58, found in 10 % of participants. Conclusion. From the results of our research, we believe that detection and identification of the broader spectrum of high-risk HPV genotypes will help to identify women who are likely to develop cervical cancer.

Keywords

• cervical cancer, hpv genotype
• hpv infection
• pcr