Melatonin reduces cadmium accumulation through cell wall fraction fixation capacity in cotton seedlings
Uzair Muhammad,
Yi Zhang,
Abid Ali,
Huazu Li,
Yifei Han,
Yue Sun,
Jielin Wang,
Wanru Wang,
Samrana Samrana,
Sarzamin Khan,
Azizullah Azizullah,
Iqbal Hussain,
Imran Ali,
Tianlun Zhao,
Shuijin Zhu
Affiliations
Uzair Muhammad
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Yi Zhang
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Abid Ali
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Department of Agriculture, Shaheed B. B. University Sheringal, Khyber Pakhtunkhwa, Pakistan
Huazu Li
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Yifei Han
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Yue Sun
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Jielin Wang
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Hainan Institute, Zhejiang University, Sanya 572025, China
Wanru Wang
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Samrana Samrana
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Department of Botany, Woman University, Swabi, Khyber Pakhtunkhwa, Pakistan
Sarzamin Khan
Department of Chemistry, University of Swabi, Anbar, Khyber Pakhtunkhwa, -23561, Pakistan
Azizullah Azizullah
Department of Botany, University of Haripur, Haripur, Khyber Pakhtunkhwa, Pakistan
Iqbal Hussain
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Imran Ali
Department of Botany, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan
Tianlun Zhao
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Hainan Institute, Zhejiang University, Sanya 572025, China; Corresponding authors.
Shuijin Zhu
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Hainan Institute, Zhejiang University, Sanya 572025, China; Corresponding authors.
Melatonin (MT) participates in plants' responses in plants’ response to cadmium (Cd) tolerance, although its work model remains elusive. Here, the function of MT in adjusting Cd accumulation in Gossypium hirsutum (CCRI12) was investigated. In this research we observed that exogenous 50 μM MT significantly reduced the Cd accumulation under 100 μМ Cd stress for 7 days of treatment in cotton seedlings. Cd markedly induced the ROS (H2O2, O2-) accumulation and elevated the expression of transporters for Cd uptake and translocation. After application of MT reduced ROS and MDA accumulation in response to high induction of antioxidant defense system; the genes encoding superoxide dismutase (GhSOD), catalase (GhCAT), and peroxidase (GhPOD), ascorbate peroxidase (GhAPX), and glutathione S-transferase (GhGST) were significantly upregulated accompanied by their level of content. Furthermore, MT down-regulated transporters for Cd uptake include Natural Resistance-Associated Macrophage Protein 1 (GhNRAMP3), Zinc/Iron-regulated transporter-like protein (GhZIP1/4/6), Heavy Metal ATPase gene (GhHMA2), and ATP-binding Cassette sub family C (GhABCC3/2). Exogenous application of MT raised the hemicelluloses, cellulose and pectin content accompanied by their expression levels, xyloglucan endotransglucosylase/hydrolase (GhXTH28/6/9/B), cellulose synthase like (GhCSLG1/2) and COBRA-like (GhCOBL4), pectin acetylesterases (GhPAE8/7), pectin methylesterases (GhPME31) which in turn increased the cell wall's binding capacity to Cd.
