Radiotherapy-Induced Skin Reactions Induce Fibrosis Mediated by TGF-β1 Cytokine
Cherley Borba Vieira de Andrade,
Isalira Peroba Rezende Ramos,
Alan César Nunes de Moraes,
Ana Lúcia Rosa do Nascimento,
Camila Salata,
Regina Coeli dos Santos Goldenberg,
Jorge José de Carvalho,
Carlos Eduardo Veloso de Almeida
Affiliations
Cherley Borba Vieira de Andrade
Translational Endocrinology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil
Isalira Peroba Rezende Ramos
Center Structural Biology and Bio-imaging (CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Alan César Nunes de Moraes
Department of Biology, Federal Fluminense University, Niterói, RJ, Brazil
Ana Lúcia Rosa do Nascimento
Ultrastructure Laboratory and Tissue Biology, Department of Histology and Embriology (UERJ), Rio de Janeiro, RJ, Brazil
Camila Salata
Radiological Sciences Laboratory, Department of Biophysics and Biometry, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil
Regina Coeli dos Santos Goldenberg
Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil
Jorge José de Carvalho
Ultrastructure Laboratory and Tissue Biology, Department of Histology and Embriology (UERJ), Rio de Janeiro, RJ, Brazil
Carlos Eduardo Veloso de Almeida
Radiological Sciences Laboratory, Department of Biophysics and Biometry, State University of Rio de Janeiro (UERJ), Rio de Janeiro, RJ, Brazil
Purpose: This study aimed to investigate radiation-induced lesions on the skin in an experimental animal model. Methods and Materials: Cutaneous wounds were induced in Wistar rats by 4 MeV energy electron beam irradiation, using a dose rate of 240 cGy/min, for 3 different doses (10 Gy, 40 Gy, and 60 Gy). The skin was observed 5, 10, and 25 days (D) after ionizing radiation exposition. Results: Infiltrate inflammatory process was observed in D5 and D10, for the 40 Gy and 60 Gy groups, and a progressive increase of transforming growth factor β1 is associated with this process. It could also be noted a mischaracterization of collagen fibers at the high-dose groups. Conclusion: It was observed that the lesions caused by ionizing radiation in rats were very similar to radiodermatitis in patients under radiotherapy treatment. Advances in Knowledge: This study is important to develop strategies to prevent radiation-induced skin reactions.
