Nature Communications (Mar 2020)
Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
- Guillaume Tetreau,
- Anne-Sophie Banneville,
- Elena A. Andreeva,
- Aaron S. Brewster,
- Mark S. Hunter,
- Raymond G. Sierra,
- Jean-Marie Teulon,
- Iris D. Young,
- Niamh Burke,
- Tilman A. Grünewald,
- Joël Beaudouin,
- Irina Snigireva,
- Maria Teresa Fernandez-Luna,
- Alister Burt,
- Hyun-Woo Park,
- Luca Signor,
- Jayesh A. Bafna,
- Rabia Sadir,
- Daphna Fenel,
- Elisabetta Boeri-Erba,
- Maria Bacia,
- Ninon Zala,
- Frédéric Laporte,
- Laurence Després,
- Martin Weik,
- Sébastien Boutet,
- Martin Rosenthal,
- Nicolas Coquelle,
- Manfred Burghammer,
- Duilio Cascio,
- Michael R. Sawaya,
- Mathias Winterhalter,
- Enrico Gratton,
- Irina Gutsche,
- Brian Federici,
- Jean-Luc Pellequer,
- Nicholas K. Sauter,
- Jacques-Philippe Colletier
Affiliations
- Guillaume Tetreau
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Anne-Sophie Banneville
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Elena A. Andreeva
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Aaron S. Brewster
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Mark S. Hunter
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Raymond G. Sierra
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Jean-Marie Teulon
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Iris D. Young
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Niamh Burke
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Tilman A. Grünewald
- European Synchrotron Radiation Facility (ESRF), BP 220
- Joël Beaudouin
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Irina Snigireva
- European Synchrotron Radiation Facility (ESRF), BP 220
- Maria Teresa Fernandez-Luna
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Alister Burt
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Hyun-Woo Park
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Luca Signor
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Jayesh A. Bafna
- Department of Life Sciences & Chemistry, Jacobs University
- Rabia Sadir
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Daphna Fenel
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Elisabetta Boeri-Erba
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Maria Bacia
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Ninon Zala
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Frédéric Laporte
- Univ. Grenoble Alpes, CNRS, LECA
- Laurence Després
- Univ. Grenoble Alpes, CNRS, LECA
- Martin Weik
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Sébastien Boutet
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Martin Rosenthal
- European Synchrotron Radiation Facility (ESRF), BP 220
- Nicolas Coquelle
- Large-Scale Structures Group, Institut Laue-Langevin
- Manfred Burghammer
- European Synchrotron Radiation Facility (ESRF), BP 220
- Duilio Cascio
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Michael R. Sawaya
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Mathias Winterhalter
- Univ. Grenoble Alpes, CNRS, LECA
- Enrico Gratton
- The Henry Samueli School of Engineering, University of California
- Irina Gutsche
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Brian Federici
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Jean-Luc Pellequer
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Nicholas K. Sauter
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Jacques-Philippe Colletier
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- DOI
- https://doi.org/10.1038/s41467-020-14894-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade.
