Association of miR-146a rs2910164 Variant with Multiple Sclerosis Disease in Hamadan Province (Iran)

Abstract

Background and Objectives: Multiple sclerosis disease (MS) is the most common autoimmune disease of the central nervous system among adolescence. Factors, such as environmental factors and genetic background are involved in the development of multiple sclerosis. Given that miRNAs play an important role in post-transcriptional regulatory levels in nerve cells and glial cells, thus, any change in their performance due to polymorphisms and eventually change in their expression level in the nervous system can pave the way for degenerative diseases, such as MS. In the present study, the role of miR-146a rs2910164 polymorphism, was investigated in the development of multiple sclerosis disease in the patients of Hamadan province.   Methods: In this case-control study, 150 patients with MS and 150 healthy individuals as control group, were selected. Genotyping was performed using Tetra ARMS PCR method. In the two study groups, Hardy-Weinberg equilibrium principle, was used to determine existence or absence of balance, Chi-square test was also used to determine the existence of relationship between the studied polymorphism and development of MS.   Results: No significant difference was observed in the distribution of dominant and recessive genotypes in patients with MS, as compared to healthy subjects.   Conclusion: Contrary to previous studies, the results of this study showed that there is no relationship between the polymorphism of miR146a rs2910164 and occurrence of MS.    

Keywords

• multiple sclerosis
• micrornas
• polymorphism
• genetics.