Effects of curcumin-coated nanomicelles on cerebral ischemia injury in rats by regulating lncRNA GAS5 and NF-κB signalling pathways

Abstract

AbstractThis work aimed to investigate the effects of curcumin (Cur) encapsulated in triblock copolymer nanomicelles on lncRNA GAS5 expression and NF-κB signalling pathway in ischemic stroke rats. Using polycaprolactone (PCL), polyethylene (mPEG), polyglutamic acid (PLG), and Cur as raw materials, Cur-PLG-mPEG-PCL nanoparticles were prepared. Its morphology, particle size, and Zeta potential were analyzed. Then seventy-two SD rats were randomly divided into control (Ctrl) group, model group, model + blank nanomicelles (M + BNM) group, model +80 mg/mL Cur (M + C) group, model +40 mg/mL Cur-nanomicelles (M + CNM (40 mg/mL)) group, and model +80 mg/mL Cur nanomicelle (M + CNM (80 mg/mL)) group. The results showed that mean particle size of Cur-PLG-mPEG-PCL was (148.27 ± 27.44) nm, and mean Zeta potential was (−1.97 ± 0.21) mV. The evaluation time of intelligent optimization algorithm based on Single Shot MultiBox Detector (SSD) was significantly shortened, and average EEG power spectrum of ischemic cerebral pawns was significantly lower than that of the control group at θ, α, β, and γ bands for 4h (p < 0.01). These results indicate that the computer intelligent algorithm can be used to evaluate the behaviour of rats, EEG can reflect the changes of cerebral cortical function, and Cur-PLG-mPEG-PCL can improve neurological function and degree of brain injury.

Keywords

• Ischemic stroke
• curcumin
• triblock copolymer nano-micelle
• long non-coding RNA growth arrest-specific transcript 5
• nuclear factor kappa-B
• optimized deep learning model