Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

Wanlu Liu; Javier Gallego-Bartolomé; Yuxing Zhou; Zhenhui Zhong; Ming Wang; Somsakul Pop Wongpalee; Jason Gardiner; Suhua Feng; Peggy Hsuanyu Kuo; Steven E. Jacobsen

doi:10.1038/s41467-021-23346-y

Nature Communications (May 2021)

Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

Wanlu Liu,
Javier Gallego-Bartolomé,
Yuxing Zhou,
Zhenhui Zhong,
Ming Wang,
Somsakul Pop Wongpalee,
Jason Gardiner,
Suhua Feng,
Peggy Hsuanyu Kuo,
Steven E. Jacobsen

Affiliations

Wanlu Liu: Department of Orthopedic of the Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University
Javier Gallego-Bartolomé: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Yuxing Zhou: Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University
Zhenhui Zhong: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Ming Wang: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Somsakul Pop Wongpalee: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Jason Gardiner: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Suhua Feng: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Peggy Hsuanyu Kuo: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles
Steven E. Jacobsen: Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles

DOI: https://doi.org/10.1038/s41467-021-23346-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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The ability to target DNA methylation to specific loci is important for both basic and applied research. Here, the authors fuse CG-specific methyltransferase SssI with an artificial zinc finger protein for DNA methylation targeting and show the chromatin features favorable for efficient gain of methylation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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