An evaluation of glycated haemoglobin eluting in zone 10 on capillary zone electrophoresis

Abstract

This is the first report of glycated haemoglobin (Hb) eluting in zone 10 on capillary zone electrophoresis (CZE). In an index case, the aberrant peak was picked up during routine haemoglobinopathy testing using CZE. Mass spectrometry (MS) revealed this aberrant peak to be glycated Hb. Prompted by this initial case, a study was undertaken and the results showed that there was a strong positive linear relationship (R2 = 0.774; ρ = 0.88) between the level of glycated Hb peak eluting in zone 10 and the HbA1c value. Furthermore, all samples with an HbA1c of 54 mmol/mol or greater had an HbA1c peak eluting in zone 10.

Keywords

• capillary zone electrophoresis
• glycated haemoglobin
• hba1c
• zone 10 peaks