Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression

Yining Zhu; Ruochen Shen; Ivan Vuong; Rebekah A. Reynolds; Melanie J. Shears; Zhi-Cheng Yao; Yizong Hu; Won June Cho; Jiayuan Kong; Sashank K. Reddy; Sean C. Murphy; Hai-Quan Mao

doi:10.1038/s41467-022-31993-y

Nature Communications (Jul 2022)

Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression

Yining Zhu,
Ruochen Shen,
Ivan Vuong,
Rebekah A. Reynolds,
Melanie J. Shears,
Zhi-Cheng Yao,
Yizong Hu,
Won June Cho,
Jiayuan Kong,
Sashank K. Reddy,
Sean C. Murphy,
Hai-Quan Mao

Affiliations

Yining Zhu: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Ruochen Shen: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Ivan Vuong: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Rebekah A. Reynolds: Department of Laboratory Medicine and Pathology, University of Washington
Melanie J. Shears: Department of Laboratory Medicine and Pathology, University of Washington
Zhi-Cheng Yao: Institute for NanoBioTechnology, Johns Hopkins University
Yizong Hu: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Won June Cho: Institute for NanoBioTechnology, Johns Hopkins University
Jiayuan Kong: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Sashank K. Reddy: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Sean C. Murphy: Department of Laboratory Medicine and Pathology, University of Washington
Hai-Quan Mao: Department of Biomedical Engineering, Johns Hopkins University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-31993-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Plasmid DNA offers extended transgene expression duration compared to mRNA technologies. Here, using a multi-step screening platform, the authors report the best performing nanoparticle formulations for liver-targeted plasmid DNA expression in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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