Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts

Abstract

Summary: Genetic manipulation of neural precursor cells is an important tool to study mechanisms underlying proliferation, fate specification, and neuron formation. The CRISPR/Cas9 system enables efficient genome editing but requires the clonal expansion of cells containing the desired mutation. Here, we describe a protocol for the effective generation of clonal mouse hippocampal neural precursor lines with CRISPR/Cas9-based gene knockouts. Edited cell lines can be used to investigate gene regulatory networks driving neuronal differentiation and for modeling of diseases that involve hippocampal neurogenesis.For complete details on the use and execution of this protocol, please refer to Pötzsch et al. (2021).

Keywords

• Cell biology
• Cell-based Assays
• Genetics
• Molecular biology
• CRISPR
• Neuroscience