Determination of hepatic cholesterol 7α-hydroxylase activity in man

G. Nicolau; S. Shefer; G. Salen; E.H. Mosbach

Journal of Lipid Research (Mar 1974)

Determination of hepatic cholesterol 7α-hydroxylase activity in man

G. Nicolau,
S. Shefer,
G. Salen,
E.H. Mosbach

Affiliations

G. Nicolau: Department of Lipid Research of the Public Health Research Institute of the City of New York, Inc., Department of Medicine, New York University Medical Center, and the Manhattan Veterans Administration Hospital, New York 10016
S. Shefer: Department of Lipid Research of the Public Health Research Institute of the City of New York, Inc., Department of Medicine, New York University Medical Center, and the Manhattan Veterans Administration Hospital, New York 10016
G. Salen: Department of Lipid Research of the Public Health Research Institute of the City of New York, Inc., Department of Medicine, New York University Medical Center, and the Manhattan Veterans Administration Hospital, New York 10016
E.H. Mosbach: Department of Lipid Research of the Public Health Research Institute of the City of New York, Inc., Department of Medicine, New York University Medical Center, and the Manhattan Veterans Administration Hospital, New York 10016

Journal volume & issue: Vol. 15, no. 2
pp. 146 – 151

Abstract

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Methods were developed to determine the activity of the microsomal enzyme cholesterol 7α-hydroxylase in human liver. The enzyme assay could be performed with as little as 20 mg of fresh liver tissue, thus making the procedure applicable to specimens obtained by percutaneous liver biopsy. Optimal assay conditions were determined and the identity and radioactive purity of the reaction product, cholest-5-ene-3β,7α-diol (7α-hydroxycholesterol) were established. Specific enzyme activity was measured in a number of patients with disorders of lipid metabolism.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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