Nature Communications (Aug 2018)

Directed evolution of CRISPR-Cas9 to increase its specificity

  • Jungjoon K. Lee,
  • Euihwan Jeong,
  • Joonsun Lee,
  • Minhee Jung,
  • Eunji Shin,
  • Young-hoon Kim,
  • Kangin Lee,
  • Inyoung Jung,
  • Daesik Kim,
  • Seokjoong Kim,
  • Jin-Soo Kim

DOI
https://doi.org/10.1038/s41467-018-05477-x
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.