Latin American Journal of Aquatic Research (Jul 2012)
Criopreservación de espermatozoides del lenguado Paralichthys adspersus Cryopreservation of flounder Paralichthys adspersus spermatozoa
Abstract
Para optimizar las técnicas de reproducción en cautiverio de lenguado Paralichthys adspersus; se elaboró una metodología para la criopreservación de sus espermatozoides. Para ello, se evaluó el efecto del dimetil sulfóxido (DMSO) como agente crioprotector en tres concentraciones diferentes (1,0; 1,5 y 2,0 M) sobre la motilidad espermática y su posterior congelación con cinco diferentes tasas: -7,5; -10; -12,5; -20 y -30°C min-1, utilizando un congelador automático programable. Los mayores porcentajes de motilidad espermática post congelamiento-descongelamiento (40,5 ± 13%) fueron obtenidos al utilizar una tasa de -10°C min-1 y DMSO sin encontrar diferencias significativas entre las tres diferentes concentraciones de DMSO (P To optimize the techniques of captive breeding of flounder Paralichthys adspersus, a methodology was developed for the cryopreservation of spermatozoa of this species. The effect on sperm motility post-thawing, using three different concentrations (1.0; 1.5 and 2.0 M) of DMSO as cryoprotective agent and five different freezing rates -7.5; -10; -12.5; -20 and -30°C min-1, with an automatic programmable freezer was evaluated. The highest percentages of post-thawing sperm motility were obtained by freezing sperm at -10°C min-1, no significant differences (P < 0.05) were founded between the three different concentrations of DMSO used. Subsequently, we evaluated the effect of a non-permeable cryo-additive (chicken egg yolk, VHG), in order to obtain an increasing of the percentage of sperm motility. We used a cryoprotectant solution including DMSO at three different concentrations adding 10% VHG v/v. The highest percentages of sperm motility (71.71 ± 13%) were obtained at the freezing rate of -10°C min-1 without significant differences between the three concentrations of the cryoprotectant solution in which the sample was incubated sperm (P < 0.05). A high significant difference between the sperm motility percentages post-thawing using DMSO and DMSO with VHG, was observed.
