Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus
Samr Kassem,
Mervat E. Hamdy,
Karim M. Selim,
Dalia M. A. Elmasry,
Momtaz A. Shahein,
Dalia M. El-Husseini
Affiliations
Samr Kassem
Nanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Mervat E. Hamdy
Genome Research Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Karim M. Selim
Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Dalia M. A. Elmasry
Nanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Momtaz A. Shahein
Virology Research Department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Dalia M. El-Husseini
Nanomaterials Research and Synthesis Unit, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza 12618, Egypt
Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV) virus. Hence, the LSDV traditional diagnostic tools faced many limitations regarding sensitivity, specificity, and cross-reactivity. Herein, we fabricated a paper-based turn-on fluorescent Molecularly Imprinted Polymer (MIP) sensor for the rapid detection of LSDV. The LSDV-MIPs sensor showed strong fluorescent intensity signal enhancement in response to the presence of the virus within minutes. Our sensor showed a limit of detection of 101 log10 TCID50/mL. Moreover, it showed significantly higher specificity to LSDV relative to other viruses, especially SPV. To our knowledge, this is the first record of a paper-based rapid detection test for LSDV depending on fluorescent turn-on behavior.
