Nature Communications (Sep 2018)
Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
- Thomas Vogl,
- Thomas Kickenweiz,
- Julia Pitzer,
- Lukas Sturmberger,
- Astrid Weninger,
- Bradley W. Biggs,
- Eva-Maria Köhler,
- Armin Baumschlager,
- Jasmin Elgin Fischer,
- Patrick Hyden,
- Marlies Wagner,
- Martina Baumann,
- Nicole Borth,
- Martina Geier,
- Parayil Kumaran Ajikumar,
- Anton Glieder
Affiliations
- Thomas Vogl
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Thomas Kickenweiz
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Julia Pitzer
- Austrian Centre of Industrial Biotechnology (ACIB GmbH)
- Lukas Sturmberger
- Austrian Centre of Industrial Biotechnology (ACIB GmbH)
- Astrid Weninger
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Bradley W. Biggs
- Manus Biosynthesis
- Eva-Maria Köhler
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Armin Baumschlager
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Jasmin Elgin Fischer
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Patrick Hyden
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Marlies Wagner
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- Martina Baumann
- Austrian Centre of Industrial Biotechnology (ACIB GmbH)
- Nicole Borth
- Austrian Centre of Industrial Biotechnology (ACIB GmbH)
- Martina Geier
- Austrian Centre of Industrial Biotechnology (ACIB GmbH)
- Parayil Kumaran Ajikumar
- Manus Biosynthesis
- Anton Glieder
- Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology
- DOI
- https://doi.org/10.1038/s41467-018-05915-w
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 13
Abstract
Classic monodirectional promoters are of limited use for multiple gene co-expression. Here the authors generate a library of 168 bidirectional promoters for the yeast K. phaffii (syn. P. pastoris) with diverse expression profiles to optimize metabolic pathway design.
