Halotolerant Plant Growth-Promoting Rhizobacteria Isolated From Saline Soil Improve Nitrogen Fixation and Alleviate Salt Stress in Rice Plants

Fiqriah Hanum Khumairah; Fiqriah Hanum Khumairah; Mieke Rochimi Setiawati; Betty Natalie Fitriatin; Tualar Simarmata; Saleh Alfaraj; Mohammad Javed Ansari; Hesham A. El Enshasy; Hesham A. El Enshasy; Hesham A. El Enshasy; R. Z. Sayyed; R. Z. Sayyed; Solmaz Najafi

doi:10.3389/fmicb.2022.905210

Frontiers in Microbiology (Jun 2022)

Halotolerant Plant Growth-Promoting Rhizobacteria Isolated From Saline Soil Improve Nitrogen Fixation and Alleviate Salt Stress in Rice Plants

Fiqriah Hanum Khumairah,
Fiqriah Hanum Khumairah,
Mieke Rochimi Setiawati,
Betty Natalie Fitriatin,
Tualar Simarmata,
Saleh Alfaraj,
Mohammad Javed Ansari,
Hesham A. El Enshasy,
Hesham A. El Enshasy,
Hesham A. El Enshasy,
R. Z. Sayyed,
R. Z. Sayyed,
Solmaz Najafi

Affiliations

Fiqriah Hanum Khumairah: Department of Soil Science, University of Padjadjaran, Jatinangor, Indonesia
Fiqriah Hanum Khumairah: Department of Forestry Management, State Agricultural Polytechnic of Samarinda, Samarinda, Indonesia
Mieke Rochimi Setiawati: Department of Soil Science, University of Padjadjaran, Jatinangor, Indonesia
Betty Natalie Fitriatin: Department of Soil Science, University of Padjadjaran, Jatinangor, Indonesia
Tualar Simarmata: Department of Soil Science, University of Padjadjaran, Jatinangor, Indonesia
Saleh Alfaraj: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Mohammad Javed Ansari: Department of Botany, Hindu College Moradabad, Mahatma Jyotiba Phule Rohilkhand University Bareilly, Bareilly, India
Hesham A. El Enshasy: Universiti Teknologi Malaysia (UTM), Institute of Bioproduct Development (IBD), Skudai, Malaysia
Hesham A. El Enshasy: Universiti Teknologi Malaysia (UTM), School of Chemical and Energy Engineering, Faculty of Engineering, Skudai, Malaysia
Hesham A. El Enshasy: City of Scientific Research and Technology Applications (SRTA), Alexandria, Egypt
R. Z. Sayyed: Department of Microbiology, PSGVP Mandal’s S I Patil Arts, G B Patel Science, and STKVS Commerce College, Shahada, India
R. Z. Sayyed: Department of Entomology, Asian PGPR Society for Sustainable Agriculture, Auburn University, Auburn, AL, United States
Solmaz Najafi: 0Department of Field Crops, Faculty of Agriculture, Van Yüzüncü Yıl University, Van, Turkey

DOI: https://doi.org/10.3389/fmicb.2022.905210
Journal volume & issue: Vol. 13

Abstract

Read online

Salinity is one of the most damaging abiotic stresses due to climate change impacts that affect the growth and yield of crops, especially in lowland rice fields and coastal areas. This research aimed to isolate potential halotolerant plant growth-promoting rhizobacteria from different rhizo-microbiome and use them as effective bioinoculants to improve rice growth under salinity stress conditions. Bioassay using rice seedlings was performed in a randomized block design consisting of 16 treatments (control and 15 bacterial isolates) with three replications. Results revealed that isolates S3, S5, and S6 gave higher shoot height, root length, and plant dry weight compared with control (without isolates). Based on molecular characteristics, isolates S3 and S5 were identified as Pseudomonas stutzeri and Klebsiella pneumonia. These isolates were able to promote rice growth under salinity stress conditions as halotolerant plant growth-promoting rhizobacteria. These three potent isolates were found to produce indole-3-acetic acid and nitrogenase.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

About the journal

Abstract

Keywords