PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides

Jeffrey S. Rush; Prakash Parajuli; Alessandro Ruda; Jian Li; Amol Arunrao Pohane; Svetlana Zamakhaeva; Mohammad M. Rahman; Jennifer C. Chang; Artemis Gogos; Cameron W. Kenner; Gérard Lambeau; Michael J. Federle; Konstantin V. Korotkov; Göran Widmalm; Natalia Korotkova

doi:10.1038/s41467-022-28257-0

Nature Communications (Feb 2022)

PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides

Jeffrey S. Rush,
Prakash Parajuli,
Alessandro Ruda,
Jian Li,
Amol Arunrao Pohane,
Svetlana Zamakhaeva,
Mohammad M. Rahman,
Jennifer C. Chang,
Artemis Gogos,
Cameron W. Kenner,
Gérard Lambeau,
Michael J. Federle,
Konstantin V. Korotkov,
Göran Widmalm,
Natalia Korotkova

Affiliations

Jeffrey S. Rush: Department of Molecular and Cellular Biochemistry, University of Kentucky
Prakash Parajuli: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky
Alessandro Ruda: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University
Jian Li: Department of Molecular and Cellular Biochemistry, University of Kentucky
Amol Arunrao Pohane: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky
Svetlana Zamakhaeva: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky
Mohammad M. Rahman: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky
Jennifer C. Chang: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
Artemis Gogos: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
Cameron W. Kenner: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky
Gérard Lambeau: Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
Michael J. Federle: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
Konstantin V. Korotkov: Department of Molecular and Cellular Biochemistry, University of Kentucky
Göran Widmalm: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University
Natalia Korotkova: Department of Molecular and Cellular Biochemistry, University of Kentucky

DOI: https://doi.org/10.1038/s41467-022-28257-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

Read online

The cell wall of the bacterial pathogen Group A Streptococcus is decorated with a polysaccharide termed GAC, which is a target for vaccine development. Here, Rush et al. characterize the linkage between GAC and peptidoglycan, and identify a protein that deacetylates the linkage and thus protects the pathogen against host cationic antimicrobial proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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