Antimicrobial effect of diode laser irradiation on the reduction of Candida albicans in the root canal

Abstract

Introduction: Laser treatment has been highly regarded for removing root canal microorganisms, which are the main causes of treatment failure. This study aimed to assess the effect of 810/980 nm diode laser irradiation on the reduction of Candida albicans (C. albicans) in the root canal. Material & Methods: In this experimental study, 25 healthy single-rooted human teeth were assigned to 20 test groups (two groups of 10), and 5 teeth were assigned to negative control groups. In the first group of the test, a rotary system and in the second group, a rotary laser diode were used to reduce the number of C. albicans. Two microliters of standard microbial suspension were added to each tooth and the samples were incubated (one week, 37°C, aerobic). Samples were taken from the teeth before and after instrumentation and cultured. The results were analyzed using Paired T-Test and T-Test. Results: The results pointed out that the number of yeasts showed a decrease of 92.2% (P<0.001) and 94.5% (P<0.001), respectively, after instrumentation with the mentioned systems compared to before. On the other hand, there was a significant difference (P<0.01) between the rotary system and rotary laser after instrumentation. Discussion & Conclusion: Both methods effectively reduced the number of C. albicans from dental root canals, and the rotary laser system exhibited a significant (P<0.01) reduction in the number of yeasts compared to the rotary method.

Keywords

• laser diode
• candida albicans
• root canal