Characterization and Quantification of the Compounds of the Ethanolic Extract from Caesalpinia ferrea Stem Bark and Evaluation of Their Mutagenic Activity
Carlos César Wyrepkowski,
Daryne Lu Maldonado Gomes da Costa,
Adilson Paulo Sinhorin,
Wagner Vilegas,
Rone Aparecido De Grandis,
Flavia Aparecida Resende,
Eliana Aparecida Varanda,
Lourdes Campaner dos Santos
Affiliations
Carlos César Wyrepkowski
Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil
Daryne Lu Maldonado Gomes da Costa
Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil
Adilson Paulo Sinhorin
Institute of Natural, Human, and Social Sciences, Mato Grosso Federal University (UFMT), Sinop 78557-267, Brazil
Wagner Vilegas
Experimental Campus of São Vicente, São Paulo State University (UNESP), São Vicente 11350-000, Brazil
Rone Aparecido De Grandis
Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil
Flavia Aparecida Resende
Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil
Eliana Aparecida Varanda
Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil
Lourdes Campaner dos Santos
Organic Chemistry Department, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, Brazil
Caesalpinia ferrea Martius has traditionally been used in Brazil for many medicinal purposes, such as the treatment of bronchitis, diabetes and wounds. Despite its use as a medicinal plant, there is still no data regarding the genotoxic effect of the stem bark. This present work aims to assess the qualitative and quantitative profiles of the ethanolic extract from the stem bark of C. ferrea and to evaluate its mutagenic activity, using a Salmonella/microsome assay for this species. As a result, a total of twenty compounds were identified by Flow Injection Analysis Electrospray Ionization Ion Trap Mass Spectrometry (FIA-ESI-IT-MS/MSn) in the ethanolic extract from the stem bark of C. ferrea. Hydrolyzable tannins predominated, principally gallic acid derivatives. The HPLC-DAD method was developed for rapid quantification of six gallic acid compounds and ellagic acid derivatives. C. ferrea is widely used in Brazil, and the absence of any mutagenic effect in the Salmonella/microsome assay is important for pharmacological purposes and the safe use of this plant.
