The Protective Effect of Olive Oil on Arsenic Induced Histological Changes in the Liver of Albino Rats

Abstract

Objective: To study the protective effect of olive oil against histological changes induced by arsenic in liver of albino rats. Study Design: Randomized control trail. Place and Duration of Study: The research was carried out from 1 to 30 November 2017 at National institute of Health, Islamabad. Materials and Methods: Forty five male adult albino rats were placed in three cages having 15 rats each. Distilled water was given to rats of control group I for 30 days. The rats of group II were given 40mg per kg per day of Sodium Arsenite dissolved in drinking water for 30 days. Rats in group III, in addition to sodium Arsenite received olive oil, 0.2ml per day for 30 days along with Sodium Arsenite. Dissection was done after 30 days and liver was dissected out for histological changes. Results: The use of olive oil improved the gross and microscopic changes induced by Arsenic in liver lobes(right lateral and left lateral) of Albino rats of group III as compared to group II rats, which received only arsenic. Among microscopic parameters, sinusoidal dilation, pyknosis and necrosis was markedly reduced by use of olive oil in group III rats whereas hemorrhage was absent in group III. Conclusion: Olive oil protects histological changes caused by arsenic in liver of Albino rats which include sinusoidal dilation, congestion, pyknosis, necrosis and haemorrhage.

Keywords

• evoo (extra virgin olive oil), sodium arsenite, oleuropein.