Biomedical Applications of Modified Carbon Glassy Electrode Sensor with Nanoparticles and Dendrimers

Abstract

We previously reported the development of a biosensor platform that is capable of measuring biometabolites and environmental sensitive species, such as peroxide and nitrate/nitrate, to concentrations in the order of ppb (parts per billion) or lower. In this investigation, we modified our platform with dendrimers to enhance its performance. Zero and second generation of dendrimers were coated on the surface of a carbon glassy electrode which was then modified with l-glutamate dehydrogenase (GDH) and α-keto glutarate. The resulting electrode was tested with ammonium solutions, concentrations ranged from 2 to 300 nM at pH 7.4; the results were satisfactory. Measurements at lower concentrations had better resolution than at higher concentrations and it is believed that the measurement limit can be lower than 2 nM. This biosensor platform was proven to be versatile and can be employed as a platform for ultrasensitive detecting devices in many biomedical and environmental applications.

Keywords

• Biosensor
• GDH
• Ammonium
• Glassy carbon electrode
• PAMAM