CHROMATOGRAPHIC CHARACTERIZATION OF BEMACID ROT DYE COMPOUNDS FOLLOWING CERIOPORUS SQUAMOSUS BIOREMEDIATION

Abstract

Fungi mediated bioremediation of industrial wastewaters containing azo-dyes has been gaining increased attention in the last years. One of the downsizes of bioremediation of azo-dyes is the possibility of resulting degradation aromatic amines, following breakage of azo-bond by microbial azoreductase. In the present study, HPLC technique was used for the analysis of Bemacid Rot (Bezema) azo-dye compounds, resulting from treatment of synthetic-dyed wastewater with Cerioporus squamosus fungal strain. C. squamosus is a basidiomycete bracket fungus, that has gained little attention towards bioremediation methods of industrial wastewaters. Chromatographic analyses were performed on three experimental sets: solutions of pure dyes dissolved in distilled water at concentration of 200 mg/L; solution of sample resulting from the biodegradation of dyes (containing nutrients from the culture media: salts, fungal cells, dye, biodegradation products of different polarities) and on a solution of the dye dissolved in the nutritive media. Analyses were conducted at five wavelengths (226nm, 243nm, 304nm, 500nm and 550nm). Major differences could be highlighted, in terms of elution profiles, retention times, number of peaks, and proportion of components. This allowed the highlighting of dye degradation compounds with lower retention times, which can indicate the degradation of the azo-dye into highly polar compounds.

Keywords

• Moving bed biofilm reactor
• fungi
• wastewater
• azo-dyes