Microfluidic live tracking and transcriptomics of cancer-immune cell doublets link intercellular proximity and gene regulation

Bianca C. T. Flores; Smriti Chawla; Ning Ma; Chad Sanada; Praveen Kumar Kujur; Rudy Yeung; Margot B. Bellon; Kyle Hukari; Brian Fowler; Mark Lynch; Ludmilla T. D. Chinen; Naveen Ramalingam; Debarka Sengupta; Stefanie S. Jeffrey

doi:10.1038/s42003-022-04205-y

Communications Biology (Nov 2022)

Microfluidic live tracking and transcriptomics of cancer-immune cell doublets link intercellular proximity and gene regulation

Bianca C. T. Flores,
Smriti Chawla,
Ning Ma,
Chad Sanada,
Praveen Kumar Kujur,
Rudy Yeung,
Margot B. Bellon,
Kyle Hukari,
Brian Fowler,
Mark Lynch,
Ludmilla T. D. Chinen,
Naveen Ramalingam,
Debarka Sengupta,
Stefanie S. Jeffrey

Affiliations

Bianca C. T. Flores: Department of Surgery, Stanford University School of Medicine
Smriti Chawla: Department of Computational Biology, Indraprastha Institute of Information Technology
Ning Ma: Department of Surgery, Stanford University School of Medicine
Chad Sanada: New Technologies Group, Fluidigm Corporation
Praveen Kumar Kujur: Department of Surgery, Stanford University School of Medicine
Rudy Yeung: New Technologies Group, Fluidigm Corporation
Margot B. Bellon: Department of Surgery, Stanford University School of Medicine
Kyle Hukari: New Technologies Group, Fluidigm Corporation
Brian Fowler: New Technologies Group, Fluidigm Corporation
Mark Lynch: New Technologies Group, Fluidigm Corporation
Ludmilla T. D. Chinen: Circulating Tumor Cells Group, A.C.Camargo Cancer Center
Naveen Ramalingam: New Technologies Group, Fluidigm Corporation
Debarka Sengupta: Department of Computational Biology, Indraprastha Institute of Information Technology
Stefanie S. Jeffrey: Department of Surgery, Stanford University School of Medicine

DOI: https://doi.org/10.1038/s42003-022-04205-y
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 8

Abstract

Read online

A microfluidic system combines the capture, co-incubation, time-lapse imaging, and gene expression profiling of doublets to measure the distances between cells and associated transcriptional profiles due to cell–cell interactions.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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