Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease
Tae-Geun Gweon,
Jong-Hyung Park,
Byung-Wook Kim,
Yang Kyu Choi,
Joon Sung Kim,
Sung Min Park,
Chang Whan Kim,
Hyung-Gil Kim,
Jun-Won Chung,
Incheon and Western Kyonggi Gastrointestinal Study
Affiliations
Tae-Geun Gweon
Division of Gastroenterology, Department of Internal Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea
Jong-Hyung Park
Department of Laboratory Animal Medicine, Konkuk University College of Veterinary Medicine, Seoul, Korea
Byung-Wook Kim
Division of Gastroenterology, Department of Internal Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea
Yang Kyu Choi
Department of Laboratory Animal Medicine, Konkuk University College of Veterinary Medicine, Seoul, Korea
Joon Sung Kim
Division of Gastroenterology, Department of Internal Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea
Sung Min Park
Division of Gastroenterology, Department of Internal Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea
Chang Whan Kim
Division of Gastroenterology, Department of Internal Medicine, Bucheon St. Mary’s Hospital, The Catholic University of Korea College of Medicine, Bucheon, Korea
Hyung-Gil Kim
Division of Gastroenterology, Department of Internal Medicine, Inha University College of Medicine, Incheon, Korea
Jun-Won Chung
Division of Gastroenterology, Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Korea
Incheon and Western Kyonggi Gastrointestinal Study
Background/AimsThe aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD).Methods : GERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups. An additional dose of rebamipide (100 mg/kg) was administered orally to the PPI+R group. Mucosal erosions, epithelial thickness, and leukocyte infiltration into the esophageal mucosa were measured in isolated esophagi 14 days after the procedure. A Western blot analysis was conducted to measure the expression of claudin-1, -3, and -4.Results : The mean surface area of mucosal erosions, epithelial thickness, and leukocyte infiltration were lower in the PPI group and the PPI+R group than in the control group. Western blot analysis revealed that the expression of claudin-3 and -4 was significantly higher in the PPI+R group than in the control group.Conclusion : sRebamipide may exert an additive effect in combination with PPI to modify the tight junction proteins of the esophageal mucosa in a rat model of GERD. This treatment might be associated with the relief of GERD symptoms.
