Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test

Abstract

ABSTRACT. Barros M.H.C., Shiomi H.H., Amorim L.S., Guimarães S.E.F., Lopes P.S., Siqueira J.B., Pinho R.O. & Guimarães J.D. [Spermatic Viability of Cryopreserved Semen of Piau swine breed analyzed by Thermo Resistant Test.] Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo Teste de Termorresistência. Revista Brasileira de Medicina Veterinária 36(2):131-136, 2014. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG 36571-000, Brasil. E-mail: [email protected] The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed (Sus scrofa), by thermo resistant test (TTR). Twenty two ejaculates from 5 Piau mature boars were collected by glove hand technique. To freezing, the ejaculates was split and submitted to three protocols (P): (P1) freezing method proposed by Furst et al. (2005), altered by diluent media, (P2) freezing method proposed by Furst et al. (2005), altered by cooled curve; and (P3) freezing method proposed by Ohata et al. (2001). After thawing, semen was submitted to TTR, been incubated at 37o C by 2 hours, motility sperm (MOT) and vigor (VIG) was analyzed at 30 minutes of interval. MOT and VIG after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%; 2.5±0.5, 2.9±0.4 and 3.4±0.4, respectively to P1, P2 and P3. The TTR results show gradually decrease of motility and vigor along 2 hours of test procedure utilized, with best average to protocol 3 at all time of analyze. The protocol 3 tested by Piau boars shows highest values in cellular semen cryopreservation.