Preparation of a trivalent oil-emulsion vaccine of inactivated Newcastle disease virus, infectious bronchitis virus and Haemophilus paragallinarum

Abstract

Monvalent inactivated oil-emulsion, Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and Haemophilus paragallinarum vaccines as well as a trivalent one (NDV + IBV + HP) were prepared. The cellular and humoral immune responses of chicken to these vaccines were evaluated by using lymphocytes blastogenesis assay, haemagglutination-inhibition, serum neutralization, tube agglutination and challenge tests. The results showed that the trivalent vaccine was safe, efficient as monovalent vaccines and protected chicken against Newcastle, Infectious bronchitis and infectious coryza

Keywords

• preparation; vaccine; trivalent; oil; emulsion; inactivated; newcastle; disease; virus; infectious; bronchitis; haemophilus; paragallinarum