Poultry Science (May 2024)

Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels

  • Lina M. Peñuela-Sierra,
  • Valmiro L. Aragão-Neto,
  • Paula Lozano-Cruz,
  • Juan Nicolas Mejia-Abaunza,
  • Muhammad Ali,
  • Joaquin Cabañas-Ojeda,
  • Yanlin Yang,
  • Maria Camila Alfaro-Wisaquillo,
  • Gustavo A. Quintana-Ospina,
  • Bindhu Lakshmibai Vasanthakumari,
  • Alexandra Wealleans,
  • Ye Lao,
  • Edgar O. Oviedo-Rondón

Journal volume & issue
Vol. 103, no. 5
p. 103546

Abstract

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ABSTRACT: Low crude protein (CP) diets can reduce nitrogen (N) excretion and costs by increasing N utilization efficiency. Exogenous proteases may further improve protein digestibility in low CP diets. This study first evaluated in vitro the efficacy of a multiprotease on amino acid (AA) release from feedstuffs and broiler feed. Later, a broiler study evaluated the effect of feeding corn-soybean meal diets containing 3 CP levels (17, 19, and 21% CP) with supplementation on top of 0 or 2,400 U/kg multiprotease on chicken growth performance, total tract CP, and ileal AA digestibilities, and energy utilization. Ross 708 male chickens were placed in 42 cages and assigned to 6 treatments resulting from a 3 × 2 factorial arrangement. Three isocaloric basal diets were formulated to reduce CP, but all diets maintained digestible Lys:CP in 5.47% and the same ideal protein profile. Data were analyzed in a completely randomized design. On average, the multiprotease increased (P 0.05) these parameters. BW gain decreased by 12.9% when N intake was reduced from 49.32 to 38.49 g/bird. Multiprotease supplementation improved (P 0.05) were detected, except for Cys digestibility (P < 0.01). In conclusion, low CP diets reduced growth performance and improved N utilization but negatively affected energy utilization efficiency. Exogenous multiprotease supplementation improved AME, AMEn, protein, ileal AA, and DM digestibility in the 21% CP diet without significantly affecting growth performance.

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