Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd (Feb 2014)
Investigating the Correlation between rs1049305 and rs10244884 Polymorphisms of AQP-1 Gene and Menorrhagia in Adolescents
Abstract
Introduction: Aquaporins (AQPs) are water channels present in the cell wall, allowing water – and occasionally other molecules – to pass the cell membrane. Their function is to maintain the internal milieu cells by regulating water and ion equilibrium. The endometrium undergoes structural changes during the menstrual cycle. If implantation does not occur at the end of each menstrual cycle, amplified endometrium is shed in absence of hormones and leads to bleeding. Excessive uterine bleeding over 80 cc in each menstrual cycle called menorrhagia, is a common gynecological disorder which causes large menstrual bleedings and reduced quality of life for those affected. Methods: The study included 37 patients with menorrhagia and 20 healthy individuals. Genomic DNA was extracted from peripheral blood and Genotypes were determined by ARMS-PCR and PCR-RFLP .Statistical analysis was performed using the SPSS program. Results: Regarding rs1049305, the C minor allele showed more frequency in patients' group (0.47 vs. 0.37). The results revealed that GG genotype presents less probable risk for menorrhagia. rs10244884 also shows the same frequency. Conclusion: It can be concluded that both variants are important in pathogenesis of menorrhagia and the results confirm the important role of Aquqporin–1 channel in menstruation as well as endometrium physiology.
