Structural and functional changes of liver’s microcirculation in a rat model of acute pancreatitis induced by sodium taurocholate

Abstract

To model an acute pancreatitis condition in rats, 50 mkl of 1 %, 2,5 % or 5 % solution of sodium taurocholat was injected to the pancreatic duct. Following 1, 4, 8, 12, 24, 48 and 72 hours post-injection, rats were euthanized to perform the histologic study of pancreatic tissue and to test the amylase level in the blood. Our results show that introduction of sodium taurocholate to the pancreatic duct results in a gradient appearance of hemorrhagic areas, inflammatory and necrotic changes from the head to the tail of pancreas. Quick dynamics towards recovery of the amylase concentration in the blood was observed after the injection, as well as recovery of he microvessels and the general tissue structure of a pancreas.

Keywords

• pancreas
• acute pancreatitis model
• rat
• microcirculation