Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)

Fabian U. Zwettler; Sebastian Reinhard; Davide Gambarotto; Toby D. M. Bell; Virginie Hamel; Paul Guichard; Markus Sauer

doi:10.1038/s41467-020-17086-8

Nature Communications (Jul 2020)

Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)

Fabian U. Zwettler,
Sebastian Reinhard,
Davide Gambarotto,
Toby D. M. Bell,
Virginie Hamel,
Paul Guichard,
Markus Sauer

Affiliations

Fabian U. Zwettler: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland
Sebastian Reinhard: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland
Davide Gambarotto: Department of Cell Biology, Sciences III, University of Geneva
Toby D. M. Bell: School of Chemistry, Monash University
Virginie Hamel: Department of Cell Biology, Sciences III, University of Geneva
Paul Guichard: Department of Cell Biology, Sciences III, University of Geneva
Markus Sauer: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland

DOI: https://doi.org/10.1038/s41467-020-17086-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Previous attempts to combine expansion microscopy (ExM) and single molecule localisation microscopy (SMLM) have proved challenging. Here the authors show that post-labelling Ex-SMLM improves labelling efficiency, reduces linkage error, and preserves ultrastructural details.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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