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Evolutionary and Expression Analysis of miR-#-5p and miR-#-3p at the miRNAs/isomiRs Levels

BioMed Research International. 2015;2015 DOI 10.1155/2015/168358

 

Journal Homepage

Journal Title: BioMed Research International

ISSN: 2314-6133 (Print); 2314-6141 (Online)

Publisher: Hindawi Limited

LCC Subject Category: Medicine

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB, XML

 

AUTHORS


Li Guo (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

Jiafeng Yu (Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, China)

Hao Yu (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

Yang Zhao (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

Shujie Chen (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

Changqing Xu (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

Feng Chen (Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 19 weeks

 

Abstract | Full Text

We mainly discussed miR-#-5p and miR-#-3p under three aspects: (1) primary evolutionary analysis of human miRNAs; (2) evolutionary analysis of miRNAs from different arms across the typical 10 vertebrates; (3) expression pattern analysis of miRNAs at the miRNA/isomiR levels using public small RNA sequencing datasets. We found that no bias can be detected between the numbers of 5p-miRNA and 3p-miRNA, while miRNAs from miR-#-5p and miR-#-3p show variable nucleotide compositions. IsomiR expression profiles from the two arms are always stable, but isomiR expressions in diseased samples are prone to show larger degree of dispersion. miR-#-5p and miR-#-3p have relative independent evolution/expression patterns and datasets of target mRNAs, which might also contribute to the phenomena of arm selection and/or arm switching. Simultaneously, miRNA/isomiR expression profiles may be regulated via arm selection and/or arm switching, and the dynamic miRNAome and isomiRome will adapt to functional and/or evolutionary pressures. A comprehensive analysis and further experimental study at the miRNA/isomiR levels are quite necessary for miRNA study.