Mouse mannose-binding lectin-A and ficolin-A inhibit lipopolysaccharide-mediated pro-inflammatory responses on mast cells

Abstract

It is unknown how soluble pattern-recognition receptors inblood, such as mannose-binding lectin (MBL) and ficolins,modulate mast cell-mediated inflammatory responses. Weinvestigate how mouse MBL-A or ficolin-A regulate mouse bonemarrow-derived mast cells (mBMMCs)-derived inflammatoryresponse against bacterial lipopolysaccharide (LPS) stimulation.LPS-mediated pro-inflammatory cytokine productions onmBMMCs obtained from Toll-like receptor4 (TLR4)-deficientmice, TLR2-defficient mice, and their wildtype, were specificallyattenuated by the addition of either mouse MBL-A orficolin-A in a dose-dependent manner. However, the inhibitoryeffects by mouse MBL-A or ficolin-A were restored by theaddition of mannose or N-acetylglucosamine, respectively.These results suggest that mouse MBL-A and ficolin-A bind toLPS via its carbohydrate-recognition domain and fibrinogen-likedomain, respectively, whereby cytokine production by LPSmediatedTLR4 in mBMMCs appears to be down-regulated,indicating that mouse MBL and ficolin may have an inhibitoryfunction toward mouse TLR4-mediated excessive inflammationon the mast cells. [BMB Reports 2013; 46(7): 376-381]

Keywords

• Ficolin
• Innate immunity
• Mannose-binding lectin
• Mast cell
• Toll-like receptor