Bio-Protocol (Apr 2023)

Isolation of Immunocomplexes from Zebrafish Brain

  • Jennifer Michel,
  • Adam Miller

DOI
https://doi.org/10.21769/BioProtoc.4646
Journal volume & issue
Vol. 13, no. 7

Abstract

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Zebrafish is an excellent model to study vertebrate neurobiology, but its synaptic components that mediate and regulate fast electrical synaptic transmission are largely unidentified. Here, we describe methods to solubilize and immunoprecipitate adult zebrafish brain homogenate under conditions to preserve electrical synapse protein complexes. The methods presented are well-suited to probe electrical synapse immunocomplexes, and potentially other brain-derived immunocomplexes, for candidate interactors from zebrafish brain.