Natural polyphenolic complex of grape wine shows protective effect on radioinduced nitrative stress in rat aorta

Abstract

We investigated L-arginine/NO system, whose key molecule is nitric oxide gene­rated in the NO-synthase reaction. Nitric oxide is an important signaling molecule in a wide range of physiological responses including vasodilatation and regulation of vascular tone. We determined NO-synthase activity, the total content of NO stable metabolites (nitrite- and nitrate-anions) and the content of 3′-nitrotyrosine-modified proteins in rats’ aorta under irradiation in a dose 30 cGy with and without consumption of concentrate of natural grape wine polyphenolic complex. It was shown that the content of 3′-nitrotyrosine-modified proteins decreased in 24 and in 72 hrs after irradiation, when the NO-synthase activity and the contents of nitrite and nitrate increased in 72 and in 168 hrs compared to control. This effect is leveled by the per os introduction of concentrate of natural polyphenolic complex of grape wine. Investigated concentrate caused a decrea­se of all studied parameters. Thus, we firstly analyzed the development of nitrative stress in rats’ aortic tissues caused by the action of radiation in a dose close to the natural background radiation. The ability of grape wine polyphenols to adjust the radioinduced nitrative stress was experimentally demonstrated.

Keywords

• low doses of radiation
• concentrate of wine polyphenolic complex
• nitric oxide
• no-synthase
• 3′-nitrotyrosine-modified proteins