Journal of Marine Science and Engineering (Jun 2024)

Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by <i>Phaeocystis globosa</i>

  • Honghong Guo,
  • Wentao Wang,
  • Jishu Han,
  • Jianan Zhu,
  • Zhongshi Wang,
  • Liyan He,
  • Xiuxian Song,
  • Zhiming Yu

DOI
https://doi.org/10.3390/jmse12060989
Journal volume & issue
Vol. 12, no. 6
p. 989

Abstract

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Phaeocystis globosa is an important harmful algal species that is globally distributed. Previous studies have indicated that P. globosa preferentially uptake nitrate, but the underlying mechanism is still unclear. To further verify this preference and reveal the underlying mechanism, the assimilation rates of nitrate and ammonium by P. globosa at different concentrations was quantitatively studied by using a nitrogen stable isotope (15N) tracer technique, and the regulatory mechanism was determined from the physiological and biochemical responses. The findings revealed that the preferential assimilation of nitrate by P. globosa was influenced by the ambient ammonium concentration. When the ambient concentration of ammonium was less than approximately 3.5 μmol·L−1, the assimilation rates of nitrate form P. globosa were as high as 1.05 × 10−5 μmol·L−1·h−1·cell−1. Even though the nitrate assimilation in P. globosa was inhibited at ammonium concentrations greater than this threshold, nitrate assimilation could not be completely suppressed. The activity of NR and the expression of related genes in P. globosa were also affected by ammonium. In addition, 15N signals originally labeled nitrate accumulated in ammonium. This indicated that P. globosa was likely to reduce excess nitrate to ammonium and subsequently release it into the substrate, which might be an important energy dissipation mechanism for P. globosa. These results expand the classical understanding of the utilization of nitrogen nutrients by marine phytoplankton, and offer valuable resources for comprehending the mechanism of harmful algal blooms.

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