Development of in vitro culture system for proliferation of protocormlike bodies of Oncidium Golden Anniversary orchid

Abstract

Oncidium orchid has high demand and economic value especially in floriculture industry. This study aimed to optimize a suitable medium for rapid proliferation of protocorm-like bodies (PLBs) of Oncidium Golden Anniversary orchid. Results from this study confirmed that ½ MS semi-solid medium displayed the highest growth index and total numbers of PLBs. Inclusion of 20g/L sucrose and 1mg/L BAP produced the highest PLB proliferation rate, while adding charcoal gave no significant effect. Half-moon PLB size contributes to the highest growth rate on using a thin cell layer (TCL) system. Hence, the best medium for Oncidium Golden Anniversary PLB proliferation was ½ MS semi-solid media supplemented with 20 g/L sucrose and 1 mg/L BAP.

Keywords

• oncidium golden anniversary
• protocorm-like bodies
• growth index
• thin cell layer