Frontiers in Physics (Mar 2021)

High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures

  • Yong Liang,
  • Yong Liang,
  • Xiaohu Chen,
  • Zhenglong Sun,
  • Gang Wen,
  • Chong Chen,
  • Libo Wang,
  • Xin Jin,
  • Jie Zhang,
  • Guang Yang,
  • Jing Gao,
  • Hui Li,
  • Hui Li,
  • Simin Li,
  • Simin Li

DOI
https://doi.org/10.3389/fphy.2021.648174
Journal volume & issue
Vol. 9

Abstract

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Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively.

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