Occult polyclonality of preclinical pancreatic cancer models drives in vitro evolution

Maria E. Monberg; Heather Geiger; Jaewon J. Lee; Roshan Sharma; Alexander Semaan; Vincent Bernard; Justin Wong; Fang Wang; Shaoheng Liang; Daniel B. Swartzlander; Bret M. Stephens; Matthew H. G. Katz; Ken Chen; Nicolas Robine; Paola A. Guerrero; Anirban Maitra

doi:10.1038/s41467-022-31376-3

Nature Communications (Jun 2022)

Occult polyclonality of preclinical pancreatic cancer models drives in vitro evolution

Maria E. Monberg,
Heather Geiger,
Jaewon J. Lee,
Roshan Sharma,
Alexander Semaan,
Vincent Bernard,
Justin Wong,
Fang Wang,
Shaoheng Liang,
Daniel B. Swartzlander,
Bret M. Stephens,
Matthew H. G. Katz,
Ken Chen,
Nicolas Robine,
Paola A. Guerrero,
Anirban Maitra

Affiliations

Maria E. Monberg: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Heather Geiger: New York Genome Center
Jaewon J. Lee: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Roshan Sharma: New York Genome Center
Alexander Semaan: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Vincent Bernard: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Justin Wong: Department of Epidemiology, The University of Texas MD Anderson Cancer Center
Fang Wang: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Shaoheng Liang: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Daniel B. Swartzlander: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Bret M. Stephens: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Matthew H. G. Katz: Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center
Ken Chen: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Nicolas Robine: New York Genome Center
Paola A. Guerrero: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
Anirban Maitra: Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center

DOI: https://doi.org/10.1038/s41467-022-31376-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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It is unclear if the molecular profiles of pancreatic ductal adenocarcinoma (PDAC) preclinical models remain stable during propagation. Here, the authors characterise clonal evolution throughout propagation in PDAC cell lines and a patient-derived organoid using single-cell genomics, transcriptomics and epigenomics.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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