Investigation of serum biochemical responses in White Leghorn layer birds following acute avian toxicity screening using indoxacarb

Abstract

The experiment was undertaken to study the effect of indoxacarb on various biochemical parameters in White Leghorn layer birds for a period of 14 days following a single oral administration. The study follows OECD guideline no. 223. White Leghorn layer birds (n=30) weighing between 1.2 to 1.6 kg were used for the study. Six birds were kept as the control group and approximately 2 ml of blood was collected in EDTA tubes on days 7 and 14 of the experiment. Biochemical parameters such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine and blood urea nitrogen (BUN) were estimated. The mean values of ALP, AST, ALT and BUN were found significantly higher on days 7 and 14 of the acute toxicity study of indoxacarb in layers as compared to that of the control group. Keywords: Biochemical, layers, indoxacarb