Characterisation of Trichodorus similis (Nematoda: Trichodoridae) associated with potato from the Czech Republic

Abstract

Trichodorus similis associated with potato in the Czech Republic was described and illustrated. This study provides additional information on morphometrical and morphological characters of T. similis and integrates morphological and genetic data obtained by species-specific polymerase chain reaction and sequencing (ITS1 and D2-D3 expansion segments of 28S rDNA). The knowledge on morphological variability and genetic diversity is extended, and a rapid and accurate molecular diagnostics was successfully applied.

Keywords

• d2-d3 rdna
• its1, morphology
• species-specific pcr, triplonchida