Characterization of the Plant-Associated Bacterial Microbiota of the Mexican Medicinal Species <em>Bouvardia ternifolia </em>
Loan Edel Villalobos-Flores,
Samuel David Espinosa-Torres,
Fernando Hernández-Quiroz,
Alberto Piña-Escobedo,
Yair Cruz-Narváez,
Francisco Velázquez-Escobar,
Roderich Süssmuth,
Jaime García-Mena
Affiliations
Loan Edel Villalobos-Flores
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, Ciudad de México 07360, Mexico
Samuel David Espinosa-Torres
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, Ciudad de México 07360, Mexico
Fernando Hernández-Quiroz
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, Ciudad de México 07360, Mexico
Alberto Piña-Escobedo
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, Ciudad de México 07360, Mexico
Yair Cruz-Narváez
Laboratorio de Posgrado de Operaciones Unitarias. Escuela Superior de Ingeniería Química e Industrias Extractivas del Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Ciudad de México 07738, Mexico
Francisco Velázquez-Escobar
Max Volmer Laboratorium für Biophysikalische Chemie Technische, Technische Universität Berlin, Universität Berlin Str. des 17. Juni 135/Sekr, PC-14 10623 Berlin, Germany
Roderich Süssmuth
Institut für Chemie, Sekr. TC 2, Department of Chemistry, Sekr. TC 2, Technische Universität Berlin, Straße des 17. Juni 124, 10623 Berlin, Germany
Jaime García-Mena
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av IPN 2508 Col Zacatenco, Ciudad de México 07360, Mexico
Though therapeutic compounds can be extracted directly from medicinal plant tissues; it is now known that many phytotherapeutic compounds are actually produced by associated microorganisms or due to their interaction with the plant. Bouvardia ternifolia is a medicinal plant that can be considered a potential source of therapeutic compounds, such as the antitumoral molecule bouvardin. We characterized the endophytic, endophytic+epiphyte and soil bacterial microbiota in the flower, leaves, stems and roots in specimens of this plant through 16S rDNA sequencing. We found that Proteobacteria followed by Actinobacteria were the most abundant bacteria phyla in this plant. On the other hand, the most representative genera living endophytically were Propionibacterium, Paraccocus, Lactobacillus, Kaistobacter, Methylobacterium and Erwinia. This study provides evidence of the composition and diversity of the bacterial communities present in soil and tissues of B. ternifolia which can be considered for subsequent analysis and understanding of the therapeutic compounds that give this species its medicinal properties.