Enhanced plasma persistence of ceftriaxone following single intramuscular injection with prior oral dosing of a mammary protective commercial herbal drug as complementary therapy

Abstract

Background: The herbal drug (Fibrosin®) is often used as supportive therapy with intramuscular ceftriaxone injection for treatment of mastitis in field condition. Method: A single oral dose of Fibrosin® (1.9 g) was given 1 h prior to single intramuscular injection of ceftriaxone at 50 mg kg−1 in 3 apparently healthy male and 3 female goats. Plasma and urine samples were collected at predetermined time intervals and ceftriaxone/ceftizoxime was analyzed by HPLC. Ceftriaxone persisted for 3 h in plasma of Fibrosin® treated healthy goats. Results: Ceftriaxone showed a distinct reabsorption phase in its dispositional profile. Mean t½ K (distribution half-life) following absorption phase and t½ K′ (elimination half-life) following re-absorption phase were 0.091±0.01 and 0.43±0.03 h respectively with a reabsorption half-life (t½Ka′) of 0.18±0.003 h. Ceftriaxone at a lower concentration (67.91±9.42 μg mL−1) was recovered at 24 h post dosing from urine. The reabsorption rate constant was 3.86±0.10 h−1 that suggested rapid intestinal reabsorption. Conclusion: Rapid intestinal absorption of ceftriaxone indicated shorter persistence in the intestine and it also exhibited enhanced plasma persistence which may help to preserve beneficial intestinal bacteria as well as prevention of antimicrobial resistance development in the intestine for which the herbal drug may be used as a supportive therapy with intramuscular injection of ceftriaxone.

Keywords

• Fibrosin®
• Complementary therapy
• Ceftriaxone
• Disposition kinetics
• Intramuscular injection