CircRNA_104565 Promoted Cell Proliferation in Papillary Thyroid Carcinoma by Sponging miR-134

Abstract

Jianming Gong,* Xiangdong Kong,* Jinhui Qi, Jiangkun Lu, Shaofeng Yuan, Ming Wu Department of Surgery, Tinglin Hospital of Jinshan District, Shanghai 201505, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ming WuDepartment of Surgery, Tinglin Hospital of Jinshan District, No. 80 Siping North Road, Jinshan District, Shanghai 201505, People’s Republic of ChinaTel + 86-18121367005Email [email protected]: Thyroid cancer is one of the most common cancers with rising incidence worldwide, and papillary thyroid carcinoma (PTC) accounts for 80– 85% of thyroid malignancy. Although it has been reported that many genes relate to the carcinogenesis of PTC, the molecular mechanisms remain mostly unclear.Methods: QRT-PCR assay was performed to detect circRNA_104565, miR-134 and ELF2 expression. CCK8 assay was exercised to examine cell proliferation. Western blot was used to detect ELF2 expression.Results: We found that circRNA_104565 was highly expressed in PTC tissue and cell and promoted cell proliferation in vitro and in vivo. In addition, circRNA_104565 promoted cell proliferation in PTC by regulating the miR-134/ELF2 axis.Conclusion: Hence, revealing the function of circRNA_104565 in PTC is important for understanding the molecular mechanism of carcinogenesis and providing new biomarkers or therapeutic targets for PTC.Keywords: circRNA_104565, cell proliferation, PTC, miR-134, ELF2

Keywords

• circrna_104565
• cell proliferation
• ptc
• mir-134
• elf2