Primers for Phylogeny Reconstruction in Bignonieae (Bignoniaceae) Using Herbarium Samples

Abstract

Premise of the study: New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. Methods and Results: Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. Conclusions: The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae.

Keywords

• Bignonieae
• herbarium specimens
• ndhF
• PepC
• phylogeny
• rpl32-trnL spacer