Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces

Abstract

Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. Methods: Grade II titanium discs (ASTM F86) received two different surface treatments: polished (S1) and cathodic cage plasma nitriding (S2). MBMMC were cultured on titanium discs in 24-well cell culture plates, at a density of 1 x 104 cells per well. After 24 h, the adhesion was evaluated using a hemocytometer. Results: The mean adhesion was greater on S2, though without statistically significant difference from S2 (p>0.05). Conclusions: It was demonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatible since it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing their adhesion.

Keywords

• Mesenchymal stem cells
• Bone marrow
• Titanium