BIO Web of Conferences (Jan 2023)

The Effect of Jasmonic Acid on The Growth of Dendrobium Stratiotes in Vitro

  • Arniputri Retna Bandriyati,
  • Purwanto Edi,
  • Handoyo Gani Cahyo,
  • Yunus Ahmad,
  • Samanhudi,
  • Purnomo Djoko,
  • Sakya Amalia Tetrani,
  • Rahayu Muji,
  • Setyawati Andriyana,
  • Sa’adah Istiqomatus

DOI
https://doi.org/10.1051/bioconf/20236901003
Journal volume & issue
Vol. 69
p. 01003

Abstract

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D. stratiotes is one of the endemic dendrobiums from Indonesia that grows in lowland rainforests. D. stratiotes is listed as a threatened orchid species due to the lack of available data, which results in a high uncertainty and insufficient information. This study aims to provide preliminary information of jasmonic acid effect on D. stratiotes growth in vitro in order to improve propagation techniques in agricultural practices. The exogenous application of jasmonic acid exerts a regulatory role on the plant's growth and development. This study was designed as a completed randomized design (CRD). VW medium was supplemented with jasmonic acid (0, 0.2, 0.8, 1, 1.5, 2 mg.L-1). There were 5 replications. The observed variable in this study includes leaves number, plant height, plant weight, roots number, root length, leaf anatomy, and root anatomy. Statistically, 1 mg.L-1 of jasmonic acid significantly influenced the plant height, leaves number, plant weight, and roots number which resulted in 10.16 cm, 6.53, 1.18 g, and 9.53, respectively.

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