Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges
Nilesh Kumar Dhakar,
Adrián Matencio,
Fabrizio Caldera,
Monica Argenziano,
Roberta Cavalli,
Chiara Dianzani,
Marco Zanetti,
José Manuel López-Nicolás,
Francesco Trotta
Affiliations
Nilesh Kumar Dhakar
Department of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, Italy
Adrián Matencio
Department of Biochemistry and Molecular Biology-A, Faculty of Biology, 13 University of Murcia - Regional Campus of International Excellence “Campus Mare 14 Nostrum”, E-30100 Murcia, Spain
Fabrizio Caldera
Department of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, Italy
Monica Argenziano
Department of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, Italy
Roberta Cavalli
Department of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, Italy
Chiara Dianzani
Department of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, Italy
Marco Zanetti
Department of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, Italy
José Manuel López-Nicolás
Department of Biochemistry and Molecular Biology-A, Faculty of Biology, 13 University of Murcia - Regional Campus of International Excellence “Campus Mare 14 Nostrum”, E-30100 Murcia, Spain
Francesco Trotta
Department of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, Italy
Resveratrol and oxyresveratrol are natural polyphenolic stilbenes with several important pharmacological activities. However, low solubility and aqueous instability are the major limitations in their drug delivery applications. In the present work, we demonstrated the encapsulation of resveratrol and oxyresveratrol with nanosponge to improve solubility and stability. Several characterization techniques were used to confirm the encapsulation of both drug molecules within the nanosponges. The high encapsulation efficiency of resveratrol (77.73%) and oxyresveratrol (80.33%) was achieved within the nanosponges. Transmission electron microscopy suggested uniform spherical size particles of resveratrol and oxyresveratrol loaded nanosponges. Compared to free drugs, better protection against UV degradation was observed for resveratrol-loaded nanosponge (2-fold) and oxyresveratrol-loaded nanosponge (3-fold). Moreover, a higher solubilization of resveratrol- and oxyresveratrol-loaded nanosponges lead to a better antioxidant activity compared to drug molecules alone. Cytotoxicity studies against DU-145 prostate cancer cell lines further suggested improved activity of both resveratrol and oxyresveratrol-loaded nanosponges without any significant toxicity of blank nanosponges.
